Review



ptfe pin woundmaker  (Sartorius AG)


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    Structured Review

    Sartorius AG ptfe pin woundmaker
    E-cadherin loss compromises cell migration of MCF10A CDH1-/-. Both isogenic cell lines were grown to confluence in complete media, wounds were generated using the 96-well <t>WoundMaker</t> and the data collected over 35 h and analyzed on the IncuCyte. a) Representative wounds on MCF10A isogenic cells under different ECM conditions at the start (immediately after wounding) and 14 h post wounding. b) The time course of cell migration was quantified using wound confluence at 1 h intervals over 35 h. MCF10A CDH1-/- cells were shown to take significantly longer in wound closing compared to wildtype cells under different coating conditions.
    Ptfe Pin Woundmaker, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ptfe pin woundmaker/product/Sartorius AG
    Average 86 stars, based on 1 article reviews
    ptfe pin woundmaker - by Bioz Stars, 2026-02
    86/100 stars

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    1) Product Images from "E-cadherin loss alters cytoskeletal organization and adhesion in non-malignant breast cells but is insufficient to induce an epithelial-mesenchymal transition"

    Article Title: E-cadherin loss alters cytoskeletal organization and adhesion in non-malignant breast cells but is insufficient to induce an epithelial-mesenchymal transition

    Journal: BMC Cancer

    doi: 10.1186/1471-2407-14-552

    E-cadherin loss compromises cell migration of MCF10A CDH1-/-. Both isogenic cell lines were grown to confluence in complete media, wounds were generated using the 96-well WoundMaker and the data collected over 35 h and analyzed on the IncuCyte. a) Representative wounds on MCF10A isogenic cells under different ECM conditions at the start (immediately after wounding) and 14 h post wounding. b) The time course of cell migration was quantified using wound confluence at 1 h intervals over 35 h. MCF10A CDH1-/- cells were shown to take significantly longer in wound closing compared to wildtype cells under different coating conditions.
    Figure Legend Snippet: E-cadherin loss compromises cell migration of MCF10A CDH1-/-. Both isogenic cell lines were grown to confluence in complete media, wounds were generated using the 96-well WoundMaker and the data collected over 35 h and analyzed on the IncuCyte. a) Representative wounds on MCF10A isogenic cells under different ECM conditions at the start (immediately after wounding) and 14 h post wounding. b) The time course of cell migration was quantified using wound confluence at 1 h intervals over 35 h. MCF10A CDH1-/- cells were shown to take significantly longer in wound closing compared to wildtype cells under different coating conditions.

    Techniques Used: Migration, Generated



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    Sartorius AG ptfe pin woundmaker
    E-cadherin loss compromises cell migration of MCF10A CDH1-/-. Both isogenic cell lines were grown to confluence in complete media, wounds were generated using the 96-well <t>WoundMaker</t> and the data collected over 35 h and analyzed on the IncuCyte. a) Representative wounds on MCF10A isogenic cells under different ECM conditions at the start (immediately after wounding) and 14 h post wounding. b) The time course of cell migration was quantified using wound confluence at 1 h intervals over 35 h. MCF10A CDH1-/- cells were shown to take significantly longer in wound closing compared to wildtype cells under different coating conditions.
    Ptfe Pin Woundmaker, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ptfe pin woundmaker/product/Sartorius AG
    Average 86 stars, based on 1 article reviews
    ptfe pin woundmaker - by Bioz Stars, 2026-02
    86/100 stars
      Buy from Supplier

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    E-cadherin loss compromises cell migration of MCF10A CDH1-/-. Both isogenic cell lines were grown to confluence in complete media, wounds were generated using the 96-well WoundMaker and the data collected over 35 h and analyzed on the IncuCyte. a) Representative wounds on MCF10A isogenic cells under different ECM conditions at the start (immediately after wounding) and 14 h post wounding. b) The time course of cell migration was quantified using wound confluence at 1 h intervals over 35 h. MCF10A CDH1-/- cells were shown to take significantly longer in wound closing compared to wildtype cells under different coating conditions.

    Journal: BMC Cancer

    Article Title: E-cadherin loss alters cytoskeletal organization and adhesion in non-malignant breast cells but is insufficient to induce an epithelial-mesenchymal transition

    doi: 10.1186/1471-2407-14-552

    Figure Lengend Snippet: E-cadherin loss compromises cell migration of MCF10A CDH1-/-. Both isogenic cell lines were grown to confluence in complete media, wounds were generated using the 96-well WoundMaker and the data collected over 35 h and analyzed on the IncuCyte. a) Representative wounds on MCF10A isogenic cells under different ECM conditions at the start (immediately after wounding) and 14 h post wounding. b) The time course of cell migration was quantified using wound confluence at 1 h intervals over 35 h. MCF10A CDH1-/- cells were shown to take significantly longer in wound closing compared to wildtype cells under different coating conditions.

    Article Snippet: Precise and reproducible wounds were generated using the 96 PTFE pin WoundMaker (Essen Bioscience) on the confluent monolayer and cells returned to the incubator where images of cells were acquired every 1 h for 35 h under phase contrast microscopy.

    Techniques: Migration, Generated